Journal: BMC Veterinary Research
Article Title: Investigating the immunological activity of the Hsp70-P113 fusion protein for Mycoplasma ovipneumoniae detection: a groundbreaking study
doi: 10.1186/s12917-024-04274-7
Figure Lengend Snippet: Western blot analysis of the reactogenicity of the recombinant protein Hsp70-P113. 1: Anti-His tag antibody as the primary antibody; 2: recombinant protein hyperimmune serum as the primary antibody (hyperimmune serum prepared by immunizing BALB/c mice with purified Hsp70-P113 recombinant protein); 3: Movi hyperimmune serum as the primary antibody (hyperimmune serum prepared by immunizing BALB/c mice with Movi whole bacterium antigen); 4. The serum of nonimmunized mice (blank control) was used as the primary antibody. M: protein marker. 1–4:HRP-labelled goat anti-mouse IgG was used as the secondary antibody
Article Snippet: The Protean program in DNAStar 7.0 software was utilized to analyse the secondary structures of the Hsp70 gene (FJ-29 strain, GenBank accession number: OP121620) and the P113 gene (ATCC 29419 strain, GenBank accession number: KR021380) of Movi, with the aim of identifying prominent antigenic regions.
Techniques: Western Blot, Recombinant, Purification, Control, Marker